Conference abstract
Anthelminthic activity of khaya grandifoliola ethanolic and aqueous extracts using an automated high-throughput method with Heligmosomoides polygyrus
Pan African Medical Journal - Conference Proceedings. 2023:17(38).04
Jun 2023.
doi: 10.11604/pamj-cp.2023.17.38.1593
Archived on: 04 Jun 2023
Contact the corresponding author
Keywords: Heligmosomoides bakeri, khaya grandifoliola, anthelmintic, helminthiases, lavicidal, ovicidal
Oral presentation
Anthelminthic activity of khaya grandifoliola ethanolic and aqueous extracts using an automated high-throughput method with Heligmosomoides polygyrus
Yamssi Cédric1,&, Yungong Misparine Kiki2, Noumedem Anangmo Christelle Nadia3
1Department of Biomedical Sciences, Faculty of Health Sciences, University of Bamenda, P.O. Box 39, Bambili, Cameroon, 2Department of Medical Laboratory Sciences, Faculty of Health Sciences, University of Bamenda, P.O. Box 39, Bambili, Cameroon, 3Department of Microbiology, Haematology and Immunology Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, P.O. Box 96, Dschang, Cameroon
&Corresponding author
Introduction: parasitic infections remain a major public health problem worldwide, particularly in developing countries. This study was aimed at evaluating the anthelminthic activities of Khaya grandifoliola using an automated high-throughput method on eggs and larvae stages of Heligmosomoides polygyrus.
Methods: fresh eggs of Heligmosomoides polygyrus were obtained from the faeces of experimentally infected mice. Embryonated eggs, L1, and L2 larval stages were obtained after 24h, 48h, and 5 days of coproculture, respectively. The Anthelminthic activity effect of the extracts against H. polygyrus was determined using an automated high-throughput method. Two different extracts were prepared (Aqueous extract and Ethanol extract). For the ovicidal activity, 100 µl of various plant extract concentrations, levamisole, and 1.5% DMSO were put in contact with 100 µl of a suspension of embryonated eggs (60 eggs) in a 96-well microplate and incubated for 24 h at 25o C in the WMicrotracker were movements were recorded every 30 minutes. The same procedure was used for the larval motility assays, where 100 µl of L1 or L2 larvae (50 Larvae) were put in contact with 100μl of various concentrations of extracts and incubated for 24 h at 25° C in a WMicrotracker movement was recorded. The anthelmintic activity was determined using the average movement of the worms in the tested product compared to the negative control (1.5% DMSO and distilled water).
Results: the highest percentage of inhibition of hatching was observed at 7.5mg/mL, with 43.3 ± 20.8% for the aqueous extracts. The highest larvicidal activity was observed at 7.5mg/mL with 90.0 ±10.0% and 95.0±5.0% inhibition for the ethanol and aqueous extracts respectively in the first larval stage. The concentration 7mg/ml had the highest mean inhibition rate of 98.3±2.9% and 83.3±2.9% respectively, for the ethanol and aqueous extracts in the second larval stage. Distilled water and 1.5% DMSO (negative control) had no effect while levamisole had 100% inhibition.
Conclusion: extracts of Khaya grandifoliola exhibited anthelminthic activity. However, in vivo-tests remain important in order to scientifically confirm the use of this plant for the treatment of helminthiasis.
Anthelminthic activity of khaya grandifoliola ethanolic and aqueous extracts using an automated high-throughput method with Heligmosomoides polygyrus
Yamssi Cédric1,&, Yungong Misparine Kiki2, Noumedem Anangmo Christelle Nadia3
1Department of Biomedical Sciences, Faculty of Health Sciences, University of Bamenda, P.O. Box 39, Bambili, Cameroon, 2Department of Medical Laboratory Sciences, Faculty of Health Sciences, University of Bamenda, P.O. Box 39, Bambili, Cameroon, 3Department of Microbiology, Haematology and Immunology Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, P.O. Box 96, Dschang, Cameroon
&Corresponding author
Introduction: parasitic infections remain a major public health problem worldwide, particularly in developing countries. This study was aimed at evaluating the anthelminthic activities of Khaya grandifoliola using an automated high-throughput method on eggs and larvae stages of Heligmosomoides polygyrus.
Methods: fresh eggs of Heligmosomoides polygyrus were obtained from the faeces of experimentally infected mice. Embryonated eggs, L1, and L2 larval stages were obtained after 24h, 48h, and 5 days of coproculture, respectively. The Anthelminthic activity effect of the extracts against H. polygyrus was determined using an automated high-throughput method. Two different extracts were prepared (Aqueous extract and Ethanol extract). For the ovicidal activity, 100 µl of various plant extract concentrations, levamisole, and 1.5% DMSO were put in contact with 100 µl of a suspension of embryonated eggs (60 eggs) in a 96-well microplate and incubated for 24 h at 25o C in the WMicrotracker were movements were recorded every 30 minutes. The same procedure was used for the larval motility assays, where 100 µl of L1 or L2 larvae (50 Larvae) were put in contact with 100μl of various concentrations of extracts and incubated for 24 h at 25° C in a WMicrotracker movement was recorded. The anthelmintic activity was determined using the average movement of the worms in the tested product compared to the negative control (1.5% DMSO and distilled water).
Results: the highest percentage of inhibition of hatching was observed at 7.5mg/mL, with 43.3 ± 20.8% for the aqueous extracts. The highest larvicidal activity was observed at 7.5mg/mL with 90.0 ±10.0% and 95.0±5.0% inhibition for the ethanol and aqueous extracts respectively in the first larval stage. The concentration 7mg/ml had the highest mean inhibition rate of 98.3±2.9% and 83.3±2.9% respectively, for the ethanol and aqueous extracts in the second larval stage. Distilled water and 1.5% DMSO (negative control) had no effect while levamisole had 100% inhibition.
Conclusion: extracts of Khaya grandifoliola exhibited anthelminthic activity. However, in vivo-tests remain important in order to scientifically confirm the use of this plant for the treatment of helminthiasis.
