Conference abstract
In vitro antiplasmodial, antioxidant, and cytotoxicity activity of Terminalia macroptera traditionally used in Cameroon against malaria
Pan African Medical Journal - Conference Proceedings. 2023:17(68).04
Jun 2023.
doi: 10.11604/pamj-cp.2023.17.68.1594
Archived on: 04 Jun 2023
Contact the corresponding author
Keywords: Antiplasmodial activity, cytotoxicity, antioxidant, plasmodium strains, T. Macroptera
Poster
In vitro antiplasmodial, antioxidant, and cytotoxicity activity of Terminalia macroptera traditionally used in Cameroon against malaria
Noumedem Anangmo Christelle Nadia1,&, Ngouyamsa Nsapkain Aboubakar Sidiki2, Yamssi Cedric3
1Department of Microbiology, Hematology and Immunology Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, P.O. Box 96, Dschang, Cameroon, 2Department of Animal Biology, Faculty of Science, University of Dschang, P.O. Box 067, Dschang, Cameroon, 3Department of Biomedical Sciences, Faculty of Health Sciences, University of Bamenda, P.O. Box 39 Bambili, Cameroon
&Corresponding author
Introduction: the alarming expansion of drug resistance, as well as the scarcity of effective antimalarial medications currently accessible, highlight the importance of finding novel antimalarial chemicals. This study was undertaken to provide baseline information on the use of Terminalia macroptera as an antimalarial agent.
Methods: aqueous and ethanol extracts of the stem bark were prepared using standard procedures. The antimalarial activity of a plant was investigated in culture using the fluorescence-based SYBR Green assay against chloroquine (CQ)-sensitive (3D7) and CQ-resistant (Dd2) Plasmodium falciparum strains. 2,2'-diphenyl-1-picrylhydrazyl free radical scavenging, nitric oxide scavenging, hydrogen peroxide scavenging, and reducing power, were studied. Cytotoxicity of the extracts was conducted against RAW 264.7 cell line and Red Blood Cells (RBCs).
Result: the aqueous and ethanolic extract of T. macroptera presented active activity with IC50 for 3D7 strains of 3.46±0,48 ðœ‡g/ml, and 4.10±0.39 ðœ‡g/ml for the aqueous and ethanolic extracts respectively. The same activity was obtained with the Dd2 strains with IC50 of 6.15±1.46 ðœ‡g/ml, and 7.47±0.03 ðœ‡g/ml for the aqueous and ethanolic extracts. The aqueous extracts showed good free radical scavenging properties. The IC50 of aqueous extracts was found to be 0.87 ðœ‡g/ml, 8,49 ðœ‡g/ml, 72,78 ðœ‡g/ml, 25,92 ðœ‡g/ml for DPPH, NO, H2O2 and FRAP respectively. For the cytotoxicity test, aqueous and ethanolic extracts of T. macroptera were nontoxic with a CC50 value of 292.55±1.35µg/ml and 223.25±2.75µg/ml for ethanolic and aqueous respectively. Regarding the cytotoxicity of the extracts on RBCs (hemolysis), the two extracts showed very low toxicity as compared to the positive control.
Conclusion: these findings suggest that; extracts of T. macroptera can serve as antimalarial agents. Further studies are required to scientifically validate the use of T. macroptera as antimalarial agent.
In vitro antiplasmodial, antioxidant, and cytotoxicity activity of Terminalia macroptera traditionally used in Cameroon against malaria
Noumedem Anangmo Christelle Nadia1,&, Ngouyamsa Nsapkain Aboubakar Sidiki2, Yamssi Cedric3
1Department of Microbiology, Hematology and Immunology Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, P.O. Box 96, Dschang, Cameroon, 2Department of Animal Biology, Faculty of Science, University of Dschang, P.O. Box 067, Dschang, Cameroon, 3Department of Biomedical Sciences, Faculty of Health Sciences, University of Bamenda, P.O. Box 39 Bambili, Cameroon
&Corresponding author
Introduction: the alarming expansion of drug resistance, as well as the scarcity of effective antimalarial medications currently accessible, highlight the importance of finding novel antimalarial chemicals. This study was undertaken to provide baseline information on the use of Terminalia macroptera as an antimalarial agent.
Methods: aqueous and ethanol extracts of the stem bark were prepared using standard procedures. The antimalarial activity of a plant was investigated in culture using the fluorescence-based SYBR Green assay against chloroquine (CQ)-sensitive (3D7) and CQ-resistant (Dd2) Plasmodium falciparum strains. 2,2'-diphenyl-1-picrylhydrazyl free radical scavenging, nitric oxide scavenging, hydrogen peroxide scavenging, and reducing power, were studied. Cytotoxicity of the extracts was conducted against RAW 264.7 cell line and Red Blood Cells (RBCs).
Result: the aqueous and ethanolic extract of T. macroptera presented active activity with IC50 for 3D7 strains of 3.46±0,48 ðœ‡g/ml, and 4.10±0.39 ðœ‡g/ml for the aqueous and ethanolic extracts respectively. The same activity was obtained with the Dd2 strains with IC50 of 6.15±1.46 ðœ‡g/ml, and 7.47±0.03 ðœ‡g/ml for the aqueous and ethanolic extracts. The aqueous extracts showed good free radical scavenging properties. The IC50 of aqueous extracts was found to be 0.87 ðœ‡g/ml, 8,49 ðœ‡g/ml, 72,78 ðœ‡g/ml, 25,92 ðœ‡g/ml for DPPH, NO, H2O2 and FRAP respectively. For the cytotoxicity test, aqueous and ethanolic extracts of T. macroptera were nontoxic with a CC50 value of 292.55±1.35µg/ml and 223.25±2.75µg/ml for ethanolic and aqueous respectively. Regarding the cytotoxicity of the extracts on RBCs (hemolysis), the two extracts showed very low toxicity as compared to the positive control.
Conclusion: these findings suggest that; extracts of T. macroptera can serve as antimalarial agents. Further studies are required to scientifically validate the use of T. macroptera as antimalarial agent.
