Conference abstract
Anti-HBc positive individuals among Hepatitis B surface antigen negative blood donor exhibits high liver failure in Mimboman Village, Yaoundé-Cameroon
Pan African Medical Journal - Conference Proceedings. 2023:18(112).03
Oct 2023.
doi: 10.11604/pamj-cp.2023.18.112.2220
Archived on: 03 Oct 2023
Contact the corresponding author
Keywords: Anti-HBcAb, HBsAg, blood donors, liver failure, ALT/AST
Oral presentation
Anti-HBc positive individuals among Hepatitis B surface antigen negative blood donor exhibits high liver failure in Mimboman Village, Yaoundé-Cameroon
Rodrigue Kamga Wouambo1,&, Nkem Mowundah L. Christelle2, Ruth Ngo Mooh3
1Division of Hepatology, Department of Medicine II, University of Leipzig Medical Centre, Leipzig, Germany, 2Department of Microbiology, Universite des Montagnes, Bangangte, Cameroon, 3Department of Biochemistry, University of Buea, Buea, Cameroon
&Corresponding author
Introduction: chronic hepatitis B diagnostic in blood transfusion implies routinely the research of the unique HBsAg in community areas. However, residual transmission of HBV may still happen due to HBsAg_negative chronically infected_blood_donors. Thus, the a need to look for other serological, virological, and biochemical_markers existing. This study was to compare the hepatic function of HBsAg_negative_blood donors according to the status of anti-HBcAb contact
Methods: a cross-sectional study was conducted for 2 months at the «Medical Health Center Mimboman Village Yaounde» targeting negative blood donors to Hepatitis B surface antigen routine diagnosis technique. The detection of Hepatitis B serological markers of contact (anti-HBcAb) in each participant’s samples was performed by an enzyme immunoassay-based technique on each HBsAg-negative blood donor. Moreover, a biochemical test of the liver function (Serum alanine transaminase and Serum aspartate transaminase) was carried out after obtaining informed consent and ethical clearance from the Institutional Ethics Committee of the Universite Des Montagnes. For p-value < 0.05 the observed difference was considered statistically significant.
Results: out of 180 HBsAg- blood donors enrolled, mean age: was 27.78±6.61years, there was a male predominance (74.44%) and the sex ratio (M/F) was about 3/1. The overall seroprevalence of the marker of contact (anti-HBc+) in the study population was 42.22% (76/180). As compared to women, males were more likely positive to anti-HBcAb (43.28%vs 39.13%men, p=0.55). The overall rate of ALT abnormal (ALT>40U/L) in HBsAg- blood donors was 16.67% (30/180) and 14.44% (26/180) was ALT/AST abnormal. The rate of liver failure (ALAT abnormal) was higher in anti-HBc+ [8.6% (6/70) vs 0.9%(1/104) anti-HBc-, p=0.01).
Conclusion: this study highlighted a high seroprevalence of the marker of contact with HBV (anti-HBc) and a high liver failure rate in HBsAg-negative blood donors positive to Anti-HBc. In the community area, Anti-HBc+ should also be coupled to HBsAg in HBV screening before blood transfusion. Anti-HBc+ individuals should be undergoing further testing including HBV DNA and liver enzyme (ALAT/AST).
Anti-HBc positive individuals among Hepatitis B surface antigen negative blood donor exhibits high liver failure in Mimboman Village, Yaoundé-Cameroon
Rodrigue Kamga Wouambo1,&, Nkem Mowundah L. Christelle2, Ruth Ngo Mooh3
1Division of Hepatology, Department of Medicine II, University of Leipzig Medical Centre, Leipzig, Germany, 2Department of Microbiology, Universite des Montagnes, Bangangte, Cameroon, 3Department of Biochemistry, University of Buea, Buea, Cameroon
&Corresponding author
Introduction: chronic hepatitis B diagnostic in blood transfusion implies routinely the research of the unique HBsAg in community areas. However, residual transmission of HBV may still happen due to HBsAg_negative chronically infected_blood_donors. Thus, the a need to look for other serological, virological, and biochemical_markers existing. This study was to compare the hepatic function of HBsAg_negative_blood donors according to the status of anti-HBcAb contact
Methods: a cross-sectional study was conducted for 2 months at the «Medical Health Center Mimboman Village Yaounde» targeting negative blood donors to Hepatitis B surface antigen routine diagnosis technique. The detection of Hepatitis B serological markers of contact (anti-HBcAb) in each participant’s samples was performed by an enzyme immunoassay-based technique on each HBsAg-negative blood donor. Moreover, a biochemical test of the liver function (Serum alanine transaminase and Serum aspartate transaminase) was carried out after obtaining informed consent and ethical clearance from the Institutional Ethics Committee of the Universite Des Montagnes. For p-value < 0.05 the observed difference was considered statistically significant.
Results: out of 180 HBsAg- blood donors enrolled, mean age: was 27.78±6.61years, there was a male predominance (74.44%) and the sex ratio (M/F) was about 3/1. The overall seroprevalence of the marker of contact (anti-HBc+) in the study population was 42.22% (76/180). As compared to women, males were more likely positive to anti-HBcAb (43.28%vs 39.13%men, p=0.55). The overall rate of ALT abnormal (ALT>40U/L) in HBsAg- blood donors was 16.67% (30/180) and 14.44% (26/180) was ALT/AST abnormal. The rate of liver failure (ALAT abnormal) was higher in anti-HBc+ [8.6% (6/70) vs 0.9%(1/104) anti-HBc-, p=0.01).
Conclusion: this study highlighted a high seroprevalence of the marker of contact with HBV (anti-HBc) and a high liver failure rate in HBsAg-negative blood donors positive to Anti-HBc. In the community area, Anti-HBc+ should also be coupled to HBsAg in HBV screening before blood transfusion. Anti-HBc+ individuals should be undergoing further testing including HBV DNA and liver enzyme (ALAT/AST).
