Conference abstract
Extended-spectrum β-lactamase (ESBL) Mediated Resistance in clinical enterobacterales isolated from hospitals settings: a cross-sectional multi-center study in West region, Cameroon
Pan African Medical Journal - Conference Proceedings. 2023:18(114).03
Oct 2023.
doi: 10.11604/pamj-cp.2023.18.114.2222
Archived on: 03 Oct 2023
Contact the corresponding author
Keywords: Multi-resistant, extended spectrum beta-lactamase, antibiotics
Oral presentation
Extended-spectrum β-lactamase (ESBL) Mediated Resistance in clinical enterobacterales isolated from hospitals settings: a cross-sectional multi-center study in West region, Cameroon
Nkengkana Omer Aurelle1,&, Founou Raspail Carrel1
1Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, Dschang, Cameroon
&Corresponding author
Introduction: antimicrobial resistance currently remains a major global public health problem. The 2017 WHO report has produced a priority list of pathogens requiring research and development of new antibiotics. Among the pathogens listed, carbapenem-resistant and extended-spectrum beta-lactamases produce Enterobacterales as a critical interest group. Our study aimed to determine the genotypic characterization of Enterobacterales isolated from clinical samples in the Dschang District hospital.
Methods: this was a cross-sectional analytic study, two laboratories were included in the sample collection during 4 months. Enterobacterales were identified using the Enterosystem Kit 18R, susceptibility testing was done and ESBL phenotypes were screened on the medium "ESBL Chrom agar". The detection of resistance genes was done by PCR methods.
Results: a total of 597 clinical samples were analyzed, and 124 Enterobacterales were identified with 20.77% of the burden. 45 ESBL-E have been identified with a prevalence of 29.61% (45/152). Most isolates have been identified from vaginal samples (44.44%) and stool (42.22%); the most frequent species were Escherichia coli (51.11%), Klebsiella pneumoniae (17.78%) and Citrobacter freundii (13.33%). A high level of resistance has been observed among E. coli with ceftazidime 86.96% and imipenem (56.52%). The most implicated genes were 84.44% for blaCTX-M and 73.33% for blaTEM.
Conclusion: the resistance of Enterobacterales to beta-lactams remains a major public health problem requiring real-time epidemiological surveillance and compliance with the recommendations of the national control strategy.
Extended-spectrum β-lactamase (ESBL) Mediated Resistance in clinical enterobacterales isolated from hospitals settings: a cross-sectional multi-center study in West region, Cameroon
Nkengkana Omer Aurelle1,&, Founou Raspail Carrel1
1Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, Dschang, Cameroon
&Corresponding author
Introduction: antimicrobial resistance currently remains a major global public health problem. The 2017 WHO report has produced a priority list of pathogens requiring research and development of new antibiotics. Among the pathogens listed, carbapenem-resistant and extended-spectrum beta-lactamases produce Enterobacterales as a critical interest group. Our study aimed to determine the genotypic characterization of Enterobacterales isolated from clinical samples in the Dschang District hospital.
Methods: this was a cross-sectional analytic study, two laboratories were included in the sample collection during 4 months. Enterobacterales were identified using the Enterosystem Kit 18R, susceptibility testing was done and ESBL phenotypes were screened on the medium "ESBL Chrom agar". The detection of resistance genes was done by PCR methods.
Results: a total of 597 clinical samples were analyzed, and 124 Enterobacterales were identified with 20.77% of the burden. 45 ESBL-E have been identified with a prevalence of 29.61% (45/152). Most isolates have been identified from vaginal samples (44.44%) and stool (42.22%); the most frequent species were Escherichia coli (51.11%), Klebsiella pneumoniae (17.78%) and Citrobacter freundii (13.33%). A high level of resistance has been observed among E. coli with ceftazidime 86.96% and imipenem (56.52%). The most implicated genes were 84.44% for blaCTX-M and 73.33% for blaTEM.
Conclusion: the resistance of Enterobacterales to beta-lactams remains a major public health problem requiring real-time epidemiological surveillance and compliance with the recommendations of the national control strategy.
