Conference abstract
Genetic pattern of the ST 61 cluster of the Cameroon family among Mycobacterium tuberculosis complex strains collected during the CANTAM I project in Cameroon
Pan African Medical Journal - Conference Proceedings. 2023:18(147).03
Oct 2023.
doi: 10.11604/pamj-cp.2023.18.147.2257
Archived on: 03 Oct 2023
Contact the corresponding author
Keywords: Genetic pattern, spoligotype ST61, MIRU/VNTR, tuberculosis
Oral presentation
Genetic pattern of the ST 61 cluster of the Cameroon family among Mycobacterium tuberculosis complex strains collected during the CANTAM I project in Cameroon
Tchoupe Alix Kevine1,&, Assam Assam Jean Paul2, Tiani Lionel Ulrich2, Andoseh Geneviève2, Ngono Ngane Rosalie Annie1, Penlap Beng Véronique2
1University of Douala, Douala, Cameroun, 2University of Yaounde I, Yaounde, Cameroon
&Corresponding author
Introduction: Tuberculosis (TB) remains one of the most outstanding diseases in global health concern. It is caused by Mycobacterium Tuberculosis Complex (MTBC) strains. ST 61, a cluster of the LAM10_CAM family is the most prevalent cluster implicated in TB transmission in Cameroon. Our study aimed at revealing the most discriminative loci applied to this cluster and investigate the genetic pattern in the ST 61 cluster samples.
Methods: we conducted a cross-sectional study of 12 months (between October 2019 and January 2020) that included 45 randomly selected specimens previously collected during the CANTAM 1 project. Following DNA extraction, amplification of 12-locus Mycobacterial Interspersed Repetitive Unit-Variable Tandem Repeat (MIRU-VNTR) analysis of MIRU40, ETRA, QUB26, Mtub39, QUB4156, MIRU26, MIRU31, MIRU16, MIRU02, MIRU04, MIRU23, and MIRU27 loci, PCR assay and gel electrophoresis were performed. The amplicon sizes, allele assignations, the allelic pattern, and the technic discriminative potential were determined using MIRU-VNTR plus web application, and a dendrogram was generated.
Results: all the Forty-five isolates of clade ST61 were analyzed with MIRU/VNTR typing and detected 26 different patterns (Haplotypes). Comprising 11 sub-clusters formed by 30 isolates and 15 unique patterns (Orphans). Thus, reporting a 45% genetic pattern, and 66,6% clustering rate. we noted loci MIRU 4, 16, 23, 27, and ETRA as highly discriminative. Whereas loci MIRU 40 and QUB 4156 were moderately discriminative and loci MIRU 2, 26, 31, Mtub 39, and Qub 26 were poorly discriminative. Overall, ETR A, MIRU 4, MIRU 16, MIRU 23, MIRU 27 were revealed as the most discriminating loci applied to the cluster ST 61.
Conclusion: the identification of the most discriminative locus ETRA, MIRU 4, MIRU 16, MIRU 23, and MIRU 27 applied to a precise cluster is a safe economic and time gain option to enhance the difference between strains at the cluster level. Our study provides the first genetic diversity of 45% applied to the cluster ST 61 of the Cameroon family among strains of M. tuberculosis complex.
Genetic pattern of the ST 61 cluster of the Cameroon family among Mycobacterium tuberculosis complex strains collected during the CANTAM I project in Cameroon
Tchoupe Alix Kevine1,&, Assam Assam Jean Paul2, Tiani Lionel Ulrich2, Andoseh Geneviève2, Ngono Ngane Rosalie Annie1, Penlap Beng Véronique2
1University of Douala, Douala, Cameroun, 2University of Yaounde I, Yaounde, Cameroon
&Corresponding author
Introduction: Tuberculosis (TB) remains one of the most outstanding diseases in global health concern. It is caused by Mycobacterium Tuberculosis Complex (MTBC) strains. ST 61, a cluster of the LAM10_CAM family is the most prevalent cluster implicated in TB transmission in Cameroon. Our study aimed at revealing the most discriminative loci applied to this cluster and investigate the genetic pattern in the ST 61 cluster samples.
Methods: we conducted a cross-sectional study of 12 months (between October 2019 and January 2020) that included 45 randomly selected specimens previously collected during the CANTAM 1 project. Following DNA extraction, amplification of 12-locus Mycobacterial Interspersed Repetitive Unit-Variable Tandem Repeat (MIRU-VNTR) analysis of MIRU40, ETRA, QUB26, Mtub39, QUB4156, MIRU26, MIRU31, MIRU16, MIRU02, MIRU04, MIRU23, and MIRU27 loci, PCR assay and gel electrophoresis were performed. The amplicon sizes, allele assignations, the allelic pattern, and the technic discriminative potential were determined using MIRU-VNTR plus web application, and a dendrogram was generated.
Results: all the Forty-five isolates of clade ST61 were analyzed with MIRU/VNTR typing and detected 26 different patterns (Haplotypes). Comprising 11 sub-clusters formed by 30 isolates and 15 unique patterns (Orphans). Thus, reporting a 45% genetic pattern, and 66,6% clustering rate. we noted loci MIRU 4, 16, 23, 27, and ETRA as highly discriminative. Whereas loci MIRU 40 and QUB 4156 were moderately discriminative and loci MIRU 2, 26, 31, Mtub 39, and Qub 26 were poorly discriminative. Overall, ETR A, MIRU 4, MIRU 16, MIRU 23, MIRU 27 were revealed as the most discriminating loci applied to the cluster ST 61.
Conclusion: the identification of the most discriminative locus ETRA, MIRU 4, MIRU 16, MIRU 23, and MIRU 27 applied to a precise cluster is a safe economic and time gain option to enhance the difference between strains at the cluster level. Our study provides the first genetic diversity of 45% applied to the cluster ST 61 of the Cameroon family among strains of M. tuberculosis complex.
