Conference abstract
First detection and co-occurrence of multiple KDR mutations in insecticide-resistant Aedes aegypti from three agro-ecosystems in Benin
Pan African Medical Journal - Conference Proceedings. 2023:18(165).03
Oct 2023.
doi: 10.11604/pamj-cp.2023.18.165.2283
Archived on: 03 Oct 2023
Contact the corresponding author
Keywords: Aedes, insecticide resistance, vector control, Benin
Oral presentation
First detection and co-occurrence of multiple KDR mutations in insecticide-resistant Aedes aegypti from three agro-ecosystems in Benin
Ateutchia Ngouanet Sandra1,&, Nanfack-Minkeu Ferdinand2, Konstantinos Mavridis3, Demanou Maurice4, John Vontas3, Djouaka Rousseau5
1University of Buea, Buea, Cameroon, 2The College of Wooster, Department of Biology, Ohio, USA, 3Molecular Entomology Group, IMBB-FORTH, Heraklion, Crete, Greece, 4Regional Yellow Fever Laboratory Coordinator World Health Organization, Inter-Country Support Team West Africa, Burkina Faso, 5International Institute of Tropical Agriculture (IITA), Cotonou, Benin
&Corresponding author
Introduction: Aedes aegypti mosquito is the main vector of arboviral diseases such as dengue. Since treatment for this disease is limited, control of the vector with the use of pyrethroid insecticides is still essential. The emergence of insecticide resistance in vectors, especially against pyrethroids, is a serious threat. The objective was to explore the resistance status to pyrethroids (permethrin) and the underlying mechanisms of Ae. Aegypti population from Benin, West Africa.
Methods: Aedes larvae were collected from April to October 2021 in three agroecosystems using oviposition cup traps once ovipositing female lay their eggs. After emergence, adults (F0) were exposed to permethrin (0.75%) following WHO bioassay protocols. TaqMan RT-qPCR assays were used to detect KDR mutations and the expression profiles of eight detoxifying genes. Statistical analyses were performed according to the Pfaffl method.
Results: a total of 1877 mosquitoes were collected comprising 1764 (93.97%) Ae. aegypti and 113 (6.02%) Ae. Albopictus. All Ae. aegypti sampled were found to be resistant to Permethrin. Co-occurrence of KDR V1016G and F1534C mutations and the triple mutant combination S989P + V1016G + F1534C were detected in Ae. aegypti. P450s genes were also significantly overexpressed.
Conclusion: this study reveals for the first time the Co-occurrence of KDR V1016G and F1534C mutations and triple mutant combination S989P + V1016G + F1534C in Ae. aegypti populations in Benin. The combination of the three kdr alleles confers high resistance to Permethrin. The role of other insecticides needs to be established to provide relevant information that should be utilized in the implementation of arbovirus vector control strategies and insecticide resistance management.
First detection and co-occurrence of multiple KDR mutations in insecticide-resistant Aedes aegypti from three agro-ecosystems in Benin
Ateutchia Ngouanet Sandra1,&, Nanfack-Minkeu Ferdinand2, Konstantinos Mavridis3, Demanou Maurice4, John Vontas3, Djouaka Rousseau5
1University of Buea, Buea, Cameroon, 2The College of Wooster, Department of Biology, Ohio, USA, 3Molecular Entomology Group, IMBB-FORTH, Heraklion, Crete, Greece, 4Regional Yellow Fever Laboratory Coordinator World Health Organization, Inter-Country Support Team West Africa, Burkina Faso, 5International Institute of Tropical Agriculture (IITA), Cotonou, Benin
&Corresponding author
Introduction: Aedes aegypti mosquito is the main vector of arboviral diseases such as dengue. Since treatment for this disease is limited, control of the vector with the use of pyrethroid insecticides is still essential. The emergence of insecticide resistance in vectors, especially against pyrethroids, is a serious threat. The objective was to explore the resistance status to pyrethroids (permethrin) and the underlying mechanisms of Ae. Aegypti population from Benin, West Africa.
Methods: Aedes larvae were collected from April to October 2021 in three agroecosystems using oviposition cup traps once ovipositing female lay their eggs. After emergence, adults (F0) were exposed to permethrin (0.75%) following WHO bioassay protocols. TaqMan RT-qPCR assays were used to detect KDR mutations and the expression profiles of eight detoxifying genes. Statistical analyses were performed according to the Pfaffl method.
Results: a total of 1877 mosquitoes were collected comprising 1764 (93.97%) Ae. aegypti and 113 (6.02%) Ae. Albopictus. All Ae. aegypti sampled were found to be resistant to Permethrin. Co-occurrence of KDR V1016G and F1534C mutations and the triple mutant combination S989P + V1016G + F1534C were detected in Ae. aegypti. P450s genes were also significantly overexpressed.
Conclusion: this study reveals for the first time the Co-occurrence of KDR V1016G and F1534C mutations and triple mutant combination S989P + V1016G + F1534C in Ae. aegypti populations in Benin. The combination of the three kdr alleles confers high resistance to Permethrin. The role of other insecticides needs to be established to provide relevant information that should be utilized in the implementation of arbovirus vector control strategies and insecticide resistance management.
