Conference abstract
Hydroethanolic extract from the root bark of Securidaca longepedunculata (Polygalaceae) displays the antileishmanial and antioxidant activity
Pan African Medical Journal - Conference Proceedings. 2023:18(179).03
Oct 2023.
doi: 10.11604/pamj-cp.2023.18.179.2302
Archived on: 03 Oct 2023
Contact the corresponding author
Keywords: S. longepedunculata, antileishmanial, antioxidant
Oral presentation
Hydroethanolic extract from the root bark of Securidaca longepedunculata (Polygalaceae) displays the antileishmanial and antioxidant activity
Kemzeu Raoul1,&, Tchokouaha Yamthe Lauve2, Lunga Paul Keilah1
1University of Yaounde I, Yaoundé, Cameroun, 2Medical Institute for Research and Study of Medicinal Plants, Douala, Cameroun
&Corresponding author
Introduction: the management of leishmaniasis is impaired by the inefficiency of the currently used drugs due to the development of resistance and the undesirable toxic effects noticed in some patients. Nature represents an excellent starting tool through medicinal plants. Thus, the current study aimed to evaluate the antileishmanial and antioxidant activity of hydroethanolic extracts from Securidaca longepedunculata (Polygalaceae).
Methods: briefly, the root bark of Securidaca longepedunculata was harvested and prepared by maceration using, hydroethanolic (H2O/EtOH 30:70 v/v). The obtained extract was further tested for their antileishmanial and cytotoxicity activity against Leishmania donovani and macrophages cell line Raw264.7 using predefined protocols.
Results: overall, the obtained results showed that the hydroethanolic and methanolic exhibited a good potency against both promastigotes (IC50s of 36.43 μg/ml) and amastigotes (IC50s of 74.62 μg/ml) forms of Leishmania donovani. This extract showed a high selectivity on the murine macrophage 264.7 cell line (SI of 3,42). These extracts finally displayed interesting DPPH (SC50 of 179.1 μg/ml) and ABTS radical scavenging abilities SC50 of 30.50 μg/ml and SC50 of 217.65 μg/ml for iron reduction (FRAP).
Conclusion: these preliminary results demonstrate the pharmacological potency of the hydroethanolic extracts from the roots bark of Securidaca longepedunculata and their potential as a source of biomolecule necessary for leishmaniasis eradication.
Hydroethanolic extract from the root bark of Securidaca longepedunculata (Polygalaceae) displays the antileishmanial and antioxidant activity
Kemzeu Raoul1,&, Tchokouaha Yamthe Lauve2, Lunga Paul Keilah1
1University of Yaounde I, Yaoundé, Cameroun, 2Medical Institute for Research and Study of Medicinal Plants, Douala, Cameroun
&Corresponding author
Introduction: the management of leishmaniasis is impaired by the inefficiency of the currently used drugs due to the development of resistance and the undesirable toxic effects noticed in some patients. Nature represents an excellent starting tool through medicinal plants. Thus, the current study aimed to evaluate the antileishmanial and antioxidant activity of hydroethanolic extracts from Securidaca longepedunculata (Polygalaceae).
Methods: briefly, the root bark of Securidaca longepedunculata was harvested and prepared by maceration using, hydroethanolic (H2O/EtOH 30:70 v/v). The obtained extract was further tested for their antileishmanial and cytotoxicity activity against Leishmania donovani and macrophages cell line Raw264.7 using predefined protocols.
Results: overall, the obtained results showed that the hydroethanolic and methanolic exhibited a good potency against both promastigotes (IC50s of 36.43 μg/ml) and amastigotes (IC50s of 74.62 μg/ml) forms of Leishmania donovani. This extract showed a high selectivity on the murine macrophage 264.7 cell line (SI of 3,42). These extracts finally displayed interesting DPPH (SC50 of 179.1 μg/ml) and ABTS radical scavenging abilities SC50 of 30.50 μg/ml and SC50 of 217.65 μg/ml for iron reduction (FRAP).
Conclusion: these preliminary results demonstrate the pharmacological potency of the hydroethanolic extracts from the roots bark of Securidaca longepedunculata and their potential as a source of biomolecule necessary for leishmaniasis eradication.
