Conference abstract
Prevalence of Escherichia coli producing extended-spectrum beta-lactamase (ESBL) driven septicaemia in children aged 0-2 years in two districts hospitals in Yaounde, Cameroon
Pan African Medical Journal - Conference Proceedings. 2023:18(46).03
Oct 2023.
doi: 10.11604/pamj-cp.2023.18.46.1991
Archived on: 03 Oct 2023
Contact the corresponding author
Keywords: Sepsis, Escherichia coli, ESBL, antibiotics, infants
Poster
Prevalence of Escherichia coli producing extended-spectrum beta-lactamase (ESBL) driven septicaemia in children aged 0-2 years in two districts hospitals in Yaounde, Cameroon
Cécile Ingrid Djuikoué1,&, Paule Dana Djouela Djoulako2, Rodrigue Kamga Wouambo3, Suzie Titsamp Lacmago4, Audrey Dayomo1, Hortense Gonsu Kamga5, Benjamin Thumamo Pokam6, Teke Apalata7
1Department of Microbiology, Université des Montagnes, Bangangté Cameroon, 2Faculty of Medicine, Sorbonne Université Paris, France, 3Division of Hepatology, Department of Medecine II, University of Leipzig, Germany, 4Department of Microbiology, Faculty of Health Sciences, Université des Montagnes, Bangangté, Cameroun, 5Bacteriology Unit, University Teaching Hospital of Yaounde, Cameroon, 6Department of Medical Laboratory Science, University of Buea, Cameroon, 7Faculty of Health Sciences & National Health Laboratory Services, Walter Sisulu University, South Africa
&Corresponding author
Introduction: septicaemia is a public health problem worldwide with a high rate of mortality among children. Epidemiological data on this phenomenon in Cameroon are still scarce. The objective was to determine the prevalence and associated factors of septicaemia due to E. coli strains producing extended-spectrum beta-lactamase (ESBL) in two hospitals in Yaoundé, Cameroon.
Méthods: a cross-sectional study was conducted on infants aged 0 to 2 years old at the consultation and neonatology care unit of two hospitals in Yaoundé (UTHY and YGOPH) from August 2019 to March 2020. Blood sample collected per infant was cultured in hemoline performance vials, and bacterial strains were identified using the Api-20 E system. An antibiogram of isolates as well as the ESBL production were performed in accordance with the recommendations of the Antibiogram committee of the French Society of Microbiology 2019. Data were analysed in Epi-Info7.0 and for p less than 0.05, the difference was statistically significant.
Résults: of the 300 children enrolled, 130 (43.33%) were blood culture positive, and E. coli. was the most prevalent (69.23% (90/130)). Then antibiotic susceptibility test revealed that 77 over 90 E. coli strains were resistant to penicillin (with 85.55% to amoxicillin), and 34.44% were producing ESBL. Factors such as immunodeficiency, being on antibiotics, and particularly taking β-lactam were significantly associated with E. coli ESBL production ([aOR = 19.93; p = 0.0001], [aOR = 1.97; p = 0.04], and [aOR = 3.54; p = 0.01], respectively). Moreover, co-resistance to aminoglycosides, quinolones, fluoroquinolones, and cotrimoxazole were also found.
Conclusion: this study highlighted a high prevalence of E. coli ESBL in blood samples of children aged 0-2 years in Yaoundé and prompts the development of more efficient strategies against E. coli ESBL-associated mortality in infants in Cameroon
Prevalence of Escherichia coli producing extended-spectrum beta-lactamase (ESBL) driven septicaemia in children aged 0-2 years in two districts hospitals in Yaounde, Cameroon
Cécile Ingrid Djuikoué1,&, Paule Dana Djouela Djoulako2, Rodrigue Kamga Wouambo3, Suzie Titsamp Lacmago4, Audrey Dayomo1, Hortense Gonsu Kamga5, Benjamin Thumamo Pokam6, Teke Apalata7
1Department of Microbiology, Université des Montagnes, Bangangté Cameroon, 2Faculty of Medicine, Sorbonne Université Paris, France, 3Division of Hepatology, Department of Medecine II, University of Leipzig, Germany, 4Department of Microbiology, Faculty of Health Sciences, Université des Montagnes, Bangangté, Cameroun, 5Bacteriology Unit, University Teaching Hospital of Yaounde, Cameroon, 6Department of Medical Laboratory Science, University of Buea, Cameroon, 7Faculty of Health Sciences & National Health Laboratory Services, Walter Sisulu University, South Africa
&Corresponding author
Introduction: septicaemia is a public health problem worldwide with a high rate of mortality among children. Epidemiological data on this phenomenon in Cameroon are still scarce. The objective was to determine the prevalence and associated factors of septicaemia due to E. coli strains producing extended-spectrum beta-lactamase (ESBL) in two hospitals in Yaoundé, Cameroon.
Méthods: a cross-sectional study was conducted on infants aged 0 to 2 years old at the consultation and neonatology care unit of two hospitals in Yaoundé (UTHY and YGOPH) from August 2019 to March 2020. Blood sample collected per infant was cultured in hemoline performance vials, and bacterial strains were identified using the Api-20 E system. An antibiogram of isolates as well as the ESBL production were performed in accordance with the recommendations of the Antibiogram committee of the French Society of Microbiology 2019. Data were analysed in Epi-Info7.0 and for p less than 0.05, the difference was statistically significant.
Résults: of the 300 children enrolled, 130 (43.33%) were blood culture positive, and E. coli. was the most prevalent (69.23% (90/130)). Then antibiotic susceptibility test revealed that 77 over 90 E. coli strains were resistant to penicillin (with 85.55% to amoxicillin), and 34.44% were producing ESBL. Factors such as immunodeficiency, being on antibiotics, and particularly taking β-lactam were significantly associated with E. coli ESBL production ([aOR = 19.93; p = 0.0001], [aOR = 1.97; p = 0.04], and [aOR = 3.54; p = 0.01], respectively). Moreover, co-resistance to aminoglycosides, quinolones, fluoroquinolones, and cotrimoxazole were also found.
Conclusion: this study highlighted a high prevalence of E. coli ESBL in blood samples of children aged 0-2 years in Yaoundé and prompts the development of more efficient strategies against E. coli ESBL-associated mortality in infants in Cameroon
