Conference abstract
Prevalence of Klebsiella pneumoniae producing carbapenemase isolated from patients attending health facilities in Yaounde and Douala, Cameroon
Pan African Medical Journal - Conference Proceedings. 2023:18(47).03
Oct 2023.
doi: 10.11604/pamj-cp.2023.18.47.1992
Archived on: 03 Oct 2023
Contact the corresponding author
Keywords: Klebsiella pneumoniae, antibiotic resistance, carbapenemases, hospitalization
Poster
Prevalence of Klebsiella pneumoniae producing carbapenemase isolated from patients attending health facilities in Yaounde and Douala, Cameroon
Cécile Ingrid Djuikoué1,&, Paule Dana Djouela Djoulako2, Farid Wega3, Marie Mbassi3, Mireille Fock4, Benjamin Thumamo Pokam5, Teke Apalata6
1Microbiology Department, Faculty of Health Sciences, Université des Montagnes, Bangangté BP 208, Cameroon, 2Faculty of Medicine, Sorbonne Université Paris, France, 3Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Cameroon, 4Bacteriology Laboratory, Yaounde Gynaeco-Obstetric and Pediatric Hospital, Yaoundé, Cameroon, 5Department of Medical Laboratory Science, Faculty of Health Sciences, University of Buea, Cameroon, 6Faculty of Health Sciences & National Health Laboratory Services, Walter Sisulu University, South Africa
&Corresponding author
Introduction: Carbapenemase-producing Enterobacteriaceae (CPE) are considered a public health concern worldwide. Klebsiella pneumoniae producing carbapenemase reduces therapeutic alternatives, causing high rates of morbidity and mortality. The objective was to determine the prevalence of Klebsiella pneumoniae producing carbapenemase isolated from patients attending hospitals located in two cities of Came-roon, namely Yaoundé and Douala.
Methods: to achieve this goal, a descriptive cross-sectional and analytical study was carried out on clinical samples. The oxidase and biochemical identification tests using Api 20 E micro gallery were performed on colonies grown on a MacConkey medium and subcultured on a nutrient agar. Drug susceptibility testing was carried out following standard procedures. The confirmation of carbapenemase production and the classification was done by inhibitory synergy tests.
Results: for 498 samples, 237 (47.6%) K. pneumoniae were isolated.There was a high resistance to penicillins and cephalosporins and a low rate resistance to Aztreonam and carbapenems (33.8% to Ertapenem, 38.36% to Imipenem). AmpC production represented 33.33% and ESBL represented 46.41%. The prevalence of K. pneumoniae producing carbapenemase was 32.07% with 68.42% of class A against 13.16% of class B and 18.42% of class D. Of the 76 carbapenemases producing K. pneumoniae isolates, 35.53% were from Yaoundé and 64.47% were from Douala. Moreover, 61.84% isolates were from hospitalized patients. Hospitalization and ESBL production were significantly associated with carbapenemase production in Klebsiella pneumoniae (p = 0.01 and 0.001 respectively).
Conclusion: the multi-resistance of Klebsiella pneumoniae to antibiotics in Cameroon has increased considera-bly over the last decade. This study highlights the threat of this resistant pathogen in our setting, especially among hospitalized patients. Attention should be paid to this bacterium in the moni-toring of antimicrobial resistance in order to curb its development in the country.
Prevalence of Klebsiella pneumoniae producing carbapenemase isolated from patients attending health facilities in Yaounde and Douala, Cameroon
Cécile Ingrid Djuikoué1,&, Paule Dana Djouela Djoulako2, Farid Wega3, Marie Mbassi3, Mireille Fock4, Benjamin Thumamo Pokam5, Teke Apalata6
1Microbiology Department, Faculty of Health Sciences, Université des Montagnes, Bangangté BP 208, Cameroon, 2Faculty of Medicine, Sorbonne Université Paris, France, 3Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Cameroon, 4Bacteriology Laboratory, Yaounde Gynaeco-Obstetric and Pediatric Hospital, Yaoundé, Cameroon, 5Department of Medical Laboratory Science, Faculty of Health Sciences, University of Buea, Cameroon, 6Faculty of Health Sciences & National Health Laboratory Services, Walter Sisulu University, South Africa
&Corresponding author
Introduction: Carbapenemase-producing Enterobacteriaceae (CPE) are considered a public health concern worldwide. Klebsiella pneumoniae producing carbapenemase reduces therapeutic alternatives, causing high rates of morbidity and mortality. The objective was to determine the prevalence of Klebsiella pneumoniae producing carbapenemase isolated from patients attending hospitals located in two cities of Came-roon, namely Yaoundé and Douala.
Methods: to achieve this goal, a descriptive cross-sectional and analytical study was carried out on clinical samples. The oxidase and biochemical identification tests using Api 20 E micro gallery were performed on colonies grown on a MacConkey medium and subcultured on a nutrient agar. Drug susceptibility testing was carried out following standard procedures. The confirmation of carbapenemase production and the classification was done by inhibitory synergy tests.
Results: for 498 samples, 237 (47.6%) K. pneumoniae were isolated.There was a high resistance to penicillins and cephalosporins and a low rate resistance to Aztreonam and carbapenems (33.8% to Ertapenem, 38.36% to Imipenem). AmpC production represented 33.33% and ESBL represented 46.41%. The prevalence of K. pneumoniae producing carbapenemase was 32.07% with 68.42% of class A against 13.16% of class B and 18.42% of class D. Of the 76 carbapenemases producing K. pneumoniae isolates, 35.53% were from Yaoundé and 64.47% were from Douala. Moreover, 61.84% isolates were from hospitalized patients. Hospitalization and ESBL production were significantly associated with carbapenemase production in Klebsiella pneumoniae (p = 0.01 and 0.001 respectively).
Conclusion: the multi-resistance of Klebsiella pneumoniae to antibiotics in Cameroon has increased considera-bly over the last decade. This study highlights the threat of this resistant pathogen in our setting, especially among hospitalized patients. Attention should be paid to this bacterium in the moni-toring of antimicrobial resistance in order to curb its development in the country.
