Conference abstract
Fractions from uvaria angolensis (Annonaceae) as promising inhibitors of Plasmodium Falciparum and HIV1 reverse transcriptase
Pan African Medical Journal - Conference Proceedings. 2023:18(78).03
Oct 2023.
doi: 10.11604/pamj-cp.2023.18.78.2032
Archived on: 03 Oct 2023
Contact the corresponding author
Keywords: Uvaria angolensis, antiplasmodial, reverse transcriptase
Poster
Fractions from uvaria angolensis (Annonaceae) as promising inhibitors of Plasmodium Falciparum and HIV1 reverse transcriptase
Ngoutane Mfopa Alvine1,&, Corona Angela2, Mbouna Jiatsa Cedrick3, Kodjoh Eloh4, Pierluigi Caboni2, Fekam Boyom Fabrice3
1Institute of Fisheries and Aquatic Sciences, University of Douala, Yabassi, Cameroon, 2Department of Life and Environmental Sciences, University of Cagliari, Italy, 3Antimicrobial and Biocontrol Agents, Laboratory for Phytobiochemistry and Medicinal Plant Studies, Faculty of Sciences, University of Yaounde I, Yaounde, Cameroon, 4Faculty of Sciences and techniques, Sanitation laboratory, Water and Environment, University of Kara, Togo
&Corresponding author
Introduction: HIV-AIDS and malaria are considered major public health concerns with high rates of mortality and morbidity particularly in Subsaharan Africa. In addition, the spread and the emergence of drug resistance reported for these pathogens stimulated the need to search for new active substances. Thus, this work aimed to carry out the phytochemical study and to evaluate the antiviral and antiplasmodial activities of Uvaria angolensis
Methods: the methanolic extracts were fractionated and their interested fractions were submitted to a qualitative analysis LC-QTOF/MS. The anti-reverse transcriptase potential associated with DNA polymerase-dependent RNA and ribonuclease H of HIV1 was carried out by fluorometry. The cytotoxicity test of these fractions has been evaluated on the A549 cell line. The antiplasmodial in vitro activity against the K1 strain of Plasmodium falciparum was performed by fluorescence using SYBR Green I. The cytotoxicity test has been evaluated on cell line Human Foreskin Fibroblast (HFF).
Results: the crude methanolic extract of U. angolensis was the most active with an IC50 of 0.62 µg/ml and 1.00µg/ml on the RNase H and DNA polymerase respectively. The solid residue 4 and aqueous fraction exhibited excellent activity with an IC50 of 0.10µg/ml and 0.29 µg/ml respectively on ribonuclease H. In addition, 2 ethanolic extracts of U. angolensis inhibited the Plasmodium parasite with IC50 values ranging from 9.98 µg/ml to 10.00 µg/ml.
Conclusion: the results showed that the extracts, fractions and sub-fractions of U. angolensis could be a potential source of compounds with anti-reverse transcriptase properties of HIV1 and antiplasmodial for further studies.
Fractions from uvaria angolensis (Annonaceae) as promising inhibitors of Plasmodium Falciparum and HIV1 reverse transcriptase
Ngoutane Mfopa Alvine1,&, Corona Angela2, Mbouna Jiatsa Cedrick3, Kodjoh Eloh4, Pierluigi Caboni2, Fekam Boyom Fabrice3
1Institute of Fisheries and Aquatic Sciences, University of Douala, Yabassi, Cameroon, 2Department of Life and Environmental Sciences, University of Cagliari, Italy, 3Antimicrobial and Biocontrol Agents, Laboratory for Phytobiochemistry and Medicinal Plant Studies, Faculty of Sciences, University of Yaounde I, Yaounde, Cameroon, 4Faculty of Sciences and techniques, Sanitation laboratory, Water and Environment, University of Kara, Togo
&Corresponding author
Introduction: HIV-AIDS and malaria are considered major public health concerns with high rates of mortality and morbidity particularly in Subsaharan Africa. In addition, the spread and the emergence of drug resistance reported for these pathogens stimulated the need to search for new active substances. Thus, this work aimed to carry out the phytochemical study and to evaluate the antiviral and antiplasmodial activities of Uvaria angolensis
Methods: the methanolic extracts were fractionated and their interested fractions were submitted to a qualitative analysis LC-QTOF/MS. The anti-reverse transcriptase potential associated with DNA polymerase-dependent RNA and ribonuclease H of HIV1 was carried out by fluorometry. The cytotoxicity test of these fractions has been evaluated on the A549 cell line. The antiplasmodial in vitro activity against the K1 strain of Plasmodium falciparum was performed by fluorescence using SYBR Green I. The cytotoxicity test has been evaluated on cell line Human Foreskin Fibroblast (HFF).
Results: the crude methanolic extract of U. angolensis was the most active with an IC50 of 0.62 µg/ml and 1.00µg/ml on the RNase H and DNA polymerase respectively. The solid residue 4 and aqueous fraction exhibited excellent activity with an IC50 of 0.10µg/ml and 0.29 µg/ml respectively on ribonuclease H. In addition, 2 ethanolic extracts of U. angolensis inhibited the Plasmodium parasite with IC50 values ranging from 9.98 µg/ml to 10.00 µg/ml.
Conclusion: the results showed that the extracts, fractions and sub-fractions of U. angolensis could be a potential source of compounds with anti-reverse transcriptase properties of HIV1 and antiplasmodial for further studies.
