Conference abstract
Frequency and antibiotic susceptibility patterns of Streptococcus agalactiae strains isolated from women in Yaounde, Cameroon
Pan African Medical Journal - Conference Proceedings. 2023:18(80).03
Oct 2023.
doi: 10.11604/pamj-cp.2023.18.80.2034
Archived on: 03 Oct 2023
Contact the corresponding author
Keywords: Antibiotic, colonisation, prevalence, GBS, resistance phenotype
Poster
Frequency and antibiotic susceptibility patterns of Streptococcus agalactiae strains isolated from women in Yaounde, Cameroon
Cécile Ingrid Djuikoué1,&, Paule Dana Djouela Djoulako2, Rodrigue Kamga Wouambo2, Rosine Yemetio Foutsa2, Dorine Ekeu Ngatcheu1, Teke Apalata3,4
1Department of Microbiology, Faculty of Health Sciences, Université des Montagnes, Bangangté, Cameroon, 2Department of Microbiology and Parasitology, Faculty of Sciences, University of Buea, Buea, Cameroon, 3National Health Laboratory Services, Cameroon, 4Faculty of Health Sciences, Walter Sisulu University, Nelson Mandela Drive, South Africa
&Corresponding author
Introduction: group B Streptococcus (GBS) is dangerous for pregnant women and their babies, whom it is responsible for early neonatal bacterial sepsis (EOS). The colonisation levels of GBS and its resistance profile to antibiotics provide important information that is useful for orienting prevention strategies. We aimed to determine the prevalence of colonisation and antibiotic resistance, including patterns of inducible resistance to clindamycin, of GBS in the city of Yaounde.
Methods: a cross-sectional study with an analytical component was carried out from 28 June to 29 August 2020 at the BIOSANTE laboratory and YGOPH. Vaginal swabs and urine were collected from 163 women. Samples were analysed using 5% defibrinated sheep blood agar and chocolate plus polyvitex agar. The isolates were identified using the morphology of the colony, Gram staining, haemolysis, catalase tests, and latex grouping tests. Antibiogram was carried out by disk diffusion method following the recommendations of the ACFSM 2019. The double disk diffusion method was used to identify isolates with clindamycin-inducible resistance. Our data were analysed with SPSS version 2.1.
Results: the results obtained showed that the overall prevalence of colonisation by GBS was 37% (57/163), or 40.3% in non-pregnant women and 59.7% in pregnant women. Pregnancy (p-value = 0.019) and earlier (from the second semester of pregnancy) gestational age (p-value = 0.025) constituted the risk factors of maternal colonisation by GBS. In addition, the strains of GBS were resistant to all 16 antibiotics tested. A D test showed that 64.7% of GBS strains were constitutively resistant to clindamycin. We also note the presence of M phenotypes.
Conclusion: As a whole, our results demonstrated that the rate of GBS colonisation in this study was similar to or higher than those in previous reports in Cameroon. All these results indicate that attention should be paid to this bacterium in the monitoring of antimicrobial resistance and the care of pregnant women and newborns.
Frequency and antibiotic susceptibility patterns of Streptococcus agalactiae strains isolated from women in Yaounde, Cameroon
Cécile Ingrid Djuikoué1,&, Paule Dana Djouela Djoulako2, Rodrigue Kamga Wouambo2, Rosine Yemetio Foutsa2, Dorine Ekeu Ngatcheu1, Teke Apalata3,4
1Department of Microbiology, Faculty of Health Sciences, Université des Montagnes, Bangangté, Cameroon, 2Department of Microbiology and Parasitology, Faculty of Sciences, University of Buea, Buea, Cameroon, 3National Health Laboratory Services, Cameroon, 4Faculty of Health Sciences, Walter Sisulu University, Nelson Mandela Drive, South Africa
&Corresponding author
Introduction: group B Streptococcus (GBS) is dangerous for pregnant women and their babies, whom it is responsible for early neonatal bacterial sepsis (EOS). The colonisation levels of GBS and its resistance profile to antibiotics provide important information that is useful for orienting prevention strategies. We aimed to determine the prevalence of colonisation and antibiotic resistance, including patterns of inducible resistance to clindamycin, of GBS in the city of Yaounde.
Methods: a cross-sectional study with an analytical component was carried out from 28 June to 29 August 2020 at the BIOSANTE laboratory and YGOPH. Vaginal swabs and urine were collected from 163 women. Samples were analysed using 5% defibrinated sheep blood agar and chocolate plus polyvitex agar. The isolates were identified using the morphology of the colony, Gram staining, haemolysis, catalase tests, and latex grouping tests. Antibiogram was carried out by disk diffusion method following the recommendations of the ACFSM 2019. The double disk diffusion method was used to identify isolates with clindamycin-inducible resistance. Our data were analysed with SPSS version 2.1.
Results: the results obtained showed that the overall prevalence of colonisation by GBS was 37% (57/163), or 40.3% in non-pregnant women and 59.7% in pregnant women. Pregnancy (p-value = 0.019) and earlier (from the second semester of pregnancy) gestational age (p-value = 0.025) constituted the risk factors of maternal colonisation by GBS. In addition, the strains of GBS were resistant to all 16 antibiotics tested. A D test showed that 64.7% of GBS strains were constitutively resistant to clindamycin. We also note the presence of M phenotypes.
Conclusion: As a whole, our results demonstrated that the rate of GBS colonisation in this study was similar to or higher than those in previous reports in Cameroon. All these results indicate that attention should be paid to this bacterium in the monitoring of antimicrobial resistance and the care of pregnant women and newborns.
