Conference abstract
Phenotypic characterization and prevalence of carbapenemase-producing pseudomonas aeruginosa isolates in six health facilities in Cameroon
Pan African Medical Journal - Conference Proceedings. 2023:18(91).03
Oct 2023.
doi: 10.11604/pamj-cp.2023.18.91.2185
Archived on: 03 Oct 2023
Contact the corresponding author
Keywords: Pseudomonas aeruginosa, antibiotic resistance, carbapenemases, hospital
Oral presentation
Phenotypic characterization and prevalence of carbapenemase-producing pseudomonas aeruginosa isolates in six health facilities in Cameroon
Ingrid Cécile Djuikoué1,&, Paule Dana Djouela Djoulako2, Rodrigue Kamga Wouambo3, Hélène Valérie Same Njanjo4, Christiane Possi Kiyang1, Feline Leina Djantou Biankeu4, Celianthe Guegang4, Andreas Tchouotou1, Benjamin D. Thumamo Pokam5, Teke Apalata6, Katy Jeannot7
1Department of Microbiology, Faculty of Health Sciences, Université des Montagnes, Bangangté, Cameroon, 2Faculty of Medicine, Sorbonne Université Paris, France, 3Division of Hepatology, Department of Medecine II, University of Leipzig, Germany, 4Estuary Academic and Strategic Institute (IUEs/Insam), Douala, Cameroon, 5Department of Medical Laboratory Sciences, Faculty of Health Sciences, University of Buea, Cameroon, 6Walter Sisulu University, South Africa, 7Faculté de Médecine-Pharmacie, Université de Bourgogne-Franche Comté, France
&Corresponding author
Introduction: Pseudomonas aeruginosa is a gram-negative opportunistic pathogen with a great ability to adapt to stress, particularly to the selective pressure of antibiotics in the hospital environment. He constitutes a real public health concern, especially in low and middle-income countries. In Cameroon, little is known about drug resistance patterns of Pseudomonas aeruginosa. The objective was to determine the prevalence of Pseudomonas aeruginosa strains producing carbapenemases in six health facilities in the Center, Littoral, and West regions of Cameroon.
Methods: an analytical cross-sectional study was conducted over four months from July to October 2021. All Pseudomonas aeruginosa or suspected strains isolated from pathological products at the laboratory of bacteriology of different health facilities were systematically collected and underwent a re-identification. In brief, After growing on cetrimide Agar and successful subculturing on nutrient agar, an oxidase test was performed on pure colonies, followed by a miniaturized biochemical identification (Api 20NE system) of bacterial suspension (0.5McFarland). Drug susceptibility testing, the detection of extended-spectrum beta-lactamases, and overproduced inducible cephalosporinases and carbapenemases were all performed according to adequate standard procedures.
Results: for 468 isolates collected, 74.14% were confirmed Pseudomonas aeruginosa after re-identification, of which 34.49% (120/347) AmpC (CAZR & C/TS) and 32.26% (112/347) ESBL. The prevalence of carbapenemase-producing P. aeruginosa (IMPR and C/TR) was 25.07% (87/347) with 17.24 (15/87) class A and 82.76%_(72/87) class B. A high rate of resistance to penicillins (piperacillin: 70.58% and ticarcillin: 60.24%) was observed. There was 34.49% resistance to ceftazidime, 30.22% to imipenem against 37.02% to meropenem, and 25.1% to ceftolozane/tazobactam (C/T). These strains also exhibited 79.57% resistance to quinolones and about 26% to aminoglycosides families. Multivariable analysis revealed that carbapenemase-producing Pseudomonas aeruginosa-related infections were significantly associated with hospitalization (p= 0.04), maternity (p=0.03), surgery (p=0.04), and intensive care wards (p=0.04).
Conclusion: This study highlighted a high burden of carbapenemase-resistant strains-producing Pseudomonas aeruginosa. Surveillance should be intensified to prevent the dissemination and spread of these strains.
Phenotypic characterization and prevalence of carbapenemase-producing pseudomonas aeruginosa isolates in six health facilities in Cameroon
Ingrid Cécile Djuikoué1,&, Paule Dana Djouela Djoulako2, Rodrigue Kamga Wouambo3, Hélène Valérie Same Njanjo4, Christiane Possi Kiyang1, Feline Leina Djantou Biankeu4, Celianthe Guegang4, Andreas Tchouotou1, Benjamin D. Thumamo Pokam5, Teke Apalata6, Katy Jeannot7
1Department of Microbiology, Faculty of Health Sciences, Université des Montagnes, Bangangté, Cameroon, 2Faculty of Medicine, Sorbonne Université Paris, France, 3Division of Hepatology, Department of Medecine II, University of Leipzig, Germany, 4Estuary Academic and Strategic Institute (IUEs/Insam), Douala, Cameroon, 5Department of Medical Laboratory Sciences, Faculty of Health Sciences, University of Buea, Cameroon, 6Walter Sisulu University, South Africa, 7Faculté de Médecine-Pharmacie, Université de Bourgogne-Franche Comté, France
&Corresponding author
Introduction: Pseudomonas aeruginosa is a gram-negative opportunistic pathogen with a great ability to adapt to stress, particularly to the selective pressure of antibiotics in the hospital environment. He constitutes a real public health concern, especially in low and middle-income countries. In Cameroon, little is known about drug resistance patterns of Pseudomonas aeruginosa. The objective was to determine the prevalence of Pseudomonas aeruginosa strains producing carbapenemases in six health facilities in the Center, Littoral, and West regions of Cameroon.
Methods: an analytical cross-sectional study was conducted over four months from July to October 2021. All Pseudomonas aeruginosa or suspected strains isolated from pathological products at the laboratory of bacteriology of different health facilities were systematically collected and underwent a re-identification. In brief, After growing on cetrimide Agar and successful subculturing on nutrient agar, an oxidase test was performed on pure colonies, followed by a miniaturized biochemical identification (Api 20NE system) of bacterial suspension (0.5McFarland). Drug susceptibility testing, the detection of extended-spectrum beta-lactamases, and overproduced inducible cephalosporinases and carbapenemases were all performed according to adequate standard procedures.
Results: for 468 isolates collected, 74.14% were confirmed Pseudomonas aeruginosa after re-identification, of which 34.49% (120/347) AmpC (CAZR & C/TS) and 32.26% (112/347) ESBL. The prevalence of carbapenemase-producing P. aeruginosa (IMPR and C/TR) was 25.07% (87/347) with 17.24 (15/87) class A and 82.76%_(72/87) class B. A high rate of resistance to penicillins (piperacillin: 70.58% and ticarcillin: 60.24%) was observed. There was 34.49% resistance to ceftazidime, 30.22% to imipenem against 37.02% to meropenem, and 25.1% to ceftolozane/tazobactam (C/T). These strains also exhibited 79.57% resistance to quinolones and about 26% to aminoglycosides families. Multivariable analysis revealed that carbapenemase-producing Pseudomonas aeruginosa-related infections were significantly associated with hospitalization (p= 0.04), maternity (p=0.03), surgery (p=0.04), and intensive care wards (p=0.04).
Conclusion: This study highlighted a high burden of carbapenemase-resistant strains-producing Pseudomonas aeruginosa. Surveillance should be intensified to prevent the dissemination and spread of these strains.
