Conference abstract
Multi-drug resistant and extended-spectrum β-lactamase producing Klebsiella pneumoniae isolated from medical devices, hospital environments, and surgical patients in West Cameroon
Pan African Medical Journal - Conference Proceedings. 2023:18(92).03
Oct 2023.
doi: 10.11604/pamj-cp.2023.18.92.2186
Archived on: 03 Oct 2023
Contact the corresponding author
Keywords: ESBL, Klebsiella pneumoniae, West Cameroon
Oral presentation
Multi-drug resistant and extended-spectrum β-lactamase producing Klebsiella pneumoniae isolated from medical devices, hospital environments, and surgical patients in West Cameroon
Ngo Mbog Homb Thérèse Espoir1,&, Founou Zangue Raspail Carrel1,2, Patrice Landry Koudoum1,2, Jessica Ravanola Zemtsa3,4, Brice Davy Dimani2,3, Aurelia Djeumako Mbossi3, Luria Leslie Founou3,4, Michel Noubom1, Bruno Kenfack1
1Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, Cameroon, 2Antimicrobial Resistance and Infectious Disease (ARID) Research Unit, Cameroon, 3Research Institute of Centre of Expertise and Biological Diagnostic of Cameroon (CEDBCAM-RI), Cameroon, 4Reproductive, Maternal, Newborn and Child Health (ReMARCH) Research Unit, Cameroon
&Corresponding author
Introduction: Hospital-acquired infections caused by MDR bacteria remain a major public health issue. Most often, these infections are treated with antibiotics belonging to the beta-lactam family, but resistance mechanisms to 3GC by the production of ESBLs are observed. The spread of Enterobacteriaceae at human-environment and animal interfaces is increasingly felt. The objective was to determine the prevalence and make a genotypic analysis of isolates of E-ESBLs from surgical patients and the environment of the District Hospital of Dschang in Cameroon.
Methods: during our descriptive and cross-sectional study; human, hospital environment, and medical device samples were analyzed. After culture and purification on EMB agar, identification was carried out with Enterosystem 18R. The AST was carried out by disc diffusion method on Müller Hinton agar and Chrome Agar ESBL was used for the isolation of ESBL-producing Enterobacteriaceae. In addition, the detection of resistance genes blaCTX-M, blaSHV, and blaTEM was done with Singleplex and Multiplex PCR while the study of the relationship of Enterobacteriaceae by construction of the phylogenetic tree was carried out using the ERIC PCR method.
Results: in sum, 34.40% (32/93) of samples from the two interfaces, were Enterobacteriaceae, of which 71.87% (23/32) produced an ESBL. All of the K. pneumoniae isolates were resistant to cefuroxime at 100% (n= 27/27) followed by amoxicillin + clavulanic acid, cefotaxime, and ceftriaxone at 96.29% (26/27) then to Gentamicin at 81.48% (n=22/27). There are high prevalence of blaCTX-M (90.47%), blaTEM (85.71%), and blaSHV (100%) genes in K. pneumoniae strains. The phylogenetic profile divided into two subgroups, shows us a great similarity (100%) between the strains of K. pneumoniae taken between operating room 2 and the bedside tables in the hospital ward.
Conclusion: this study shows that there is cross-transmission of Enterobacteriaceae strains between surgical patients and the hospital environment. It is therefore imperative, even very urgent, to implement the “One Health” concept as a measure for the prevention and control of bacterial infections.
Multi-drug resistant and extended-spectrum β-lactamase producing Klebsiella pneumoniae isolated from medical devices, hospital environments, and surgical patients in West Cameroon
Ngo Mbog Homb Thérèse Espoir1,&, Founou Zangue Raspail Carrel1,2, Patrice Landry Koudoum1,2, Jessica Ravanola Zemtsa3,4, Brice Davy Dimani2,3, Aurelia Djeumako Mbossi3, Luria Leslie Founou3,4, Michel Noubom1, Bruno Kenfack1
1Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, Cameroon, 2Antimicrobial Resistance and Infectious Disease (ARID) Research Unit, Cameroon, 3Research Institute of Centre of Expertise and Biological Diagnostic of Cameroon (CEDBCAM-RI), Cameroon, 4Reproductive, Maternal, Newborn and Child Health (ReMARCH) Research Unit, Cameroon
&Corresponding author
Introduction: Hospital-acquired infections caused by MDR bacteria remain a major public health issue. Most often, these infections are treated with antibiotics belonging to the beta-lactam family, but resistance mechanisms to 3GC by the production of ESBLs are observed. The spread of Enterobacteriaceae at human-environment and animal interfaces is increasingly felt. The objective was to determine the prevalence and make a genotypic analysis of isolates of E-ESBLs from surgical patients and the environment of the District Hospital of Dschang in Cameroon.
Methods: during our descriptive and cross-sectional study; human, hospital environment, and medical device samples were analyzed. After culture and purification on EMB agar, identification was carried out with Enterosystem 18R. The AST was carried out by disc diffusion method on Müller Hinton agar and Chrome Agar ESBL was used for the isolation of ESBL-producing Enterobacteriaceae. In addition, the detection of resistance genes blaCTX-M, blaSHV, and blaTEM was done with Singleplex and Multiplex PCR while the study of the relationship of Enterobacteriaceae by construction of the phylogenetic tree was carried out using the ERIC PCR method.
Results: in sum, 34.40% (32/93) of samples from the two interfaces, were Enterobacteriaceae, of which 71.87% (23/32) produced an ESBL. All of the K. pneumoniae isolates were resistant to cefuroxime at 100% (n= 27/27) followed by amoxicillin + clavulanic acid, cefotaxime, and ceftriaxone at 96.29% (26/27) then to Gentamicin at 81.48% (n=22/27). There are high prevalence of blaCTX-M (90.47%), blaTEM (85.71%), and blaSHV (100%) genes in K. pneumoniae strains. The phylogenetic profile divided into two subgroups, shows us a great similarity (100%) between the strains of K. pneumoniae taken between operating room 2 and the bedside tables in the hospital ward.
Conclusion: this study shows that there is cross-transmission of Enterobacteriaceae strains between surgical patients and the hospital environment. It is therefore imperative, even very urgent, to implement the “One Health” concept as a measure for the prevention and control of bacterial infections.
