Conference abstract
Extended-Spectrum β-lactamase producing pathogenic K. pneumoniae isolated from children under five years with and without diarrhea in Yaoundé Cameroon: comparative case control study
Pan African Medical Journal - Conference Proceedings. 2023:18(97).03
Oct 2023.
doi: 10.11604/pamj-cp.2023.18.97.2192
Archived on: 03 Oct 2023
Contact the corresponding author
Keywords: Antibiotic resistance, Klebsiella pneumonia, ESBL
Oral presentation
Extended-Spectrum β-lactamase producing pathogenic K. pneumoniae isolated from children under five years with and without diarrhea in Yaoundé Cameroon: comparative case control study
Ngantene Mounandi Sephora1,&, Founou Zangue Raspail Carrel2, Jessica Ravanola Zemtsa2,3, Brice Davy Dimani2, Luria Leslie Founou2,3, Kamga Henri Fouamno1
1Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, Cameroon, 2Centre d´Expertise de Diagnostic Biologique du Cameroun (CEDBCAM-RI), Yaounde, Cameroun, 3Reproductive, Maternal, Newborn and Child Health (ReMARCH) Research Unit, Yaounde, Cameroon, 4Pediatric, Obstetrics and Gynecology Hospital of Douala, Douala, Cameroon
&Corresponding author
Introduction: extended-spectrum ß-lactamase-producing Enterobacterales (ESBL-E) are an important threat in hospital and community settings. This study aimed to determine the genotypically characterized ESBL-producing Klebsiella pneumoniae (ESBL-Kp) isolated from the stool of children under five years of age with gastroenteritis. The objective was to determine the genotypically characterized ESBL-producing Klebsiella pneumoniae isolated from the stool of children under five years of age with gastroenteritis.
Methods: a prospective and cross-sectional study was carried out at the Mother and Child Center of the Chantal Biya Foundation in Yaoundé. Data were collected through a questionnaire on socio-demographic characteristics. Stool samples were collected from children 0 to 5 years of age and grown on Mac Conkey agar containing purple crystal. Growing colonies were identified with Enterosystem 18R. A double screening for ESBL-Kp was performed by disc diffusion and culture on CHROMagar™ ESBL chromogenic agar. Antimicrobial susceptibility was performed by Kirby-Bauer disc diffusion. Conventional polymerase chain reaction was used to detect genes encoding ESBL production (blaCTX-M, blaTEM, blaSHV).
Results: the prevalence of ESBL-Kp in children was 89.83% (53/59). The majority of isolates were resistant to cefuroxime (98.11%%), amoxicillin-clavulanic acid (96.22%), ceftriaxone (90.56%), cefotaxime (88.67%) and ceftazidime (83.01%). However, most isolates were sensitive to chloramphenicol ( 75.47%), followed by ciprofloxacin (69.81%), levofloxacin (62.26%), and tobramycin (60.37%). A 60.37% prevalence of ESBL-Kp were concomitantly multidrug resistant. The prevalence of antibiotic resistance genes was high for all tested ß-lactamase genes including blaCTX-M (92.45%) and blaTEM (84.90%) compared to blaSHV (77.35%).
Conclusion: the high prevalence of ESBL-Kp and multidrug-resistant Kp among children under five evidenced in this study is concerning and particularly unexpected for this vulnerable population. These results suggest irrational use of antibiotics and underscore the need for improved routine surveillance, antimicrobial stewardship, and infection prevention measures in hospital and community settings to reduce the spread of these resistant bacteria and potential adverse outcomes.
Extended-Spectrum β-lactamase producing pathogenic K. pneumoniae isolated from children under five years with and without diarrhea in Yaoundé Cameroon: comparative case control study
Ngantene Mounandi Sephora1,&, Founou Zangue Raspail Carrel2, Jessica Ravanola Zemtsa2,3, Brice Davy Dimani2, Luria Leslie Founou2,3, Kamga Henri Fouamno1
1Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, Cameroon, 2Centre d´Expertise de Diagnostic Biologique du Cameroun (CEDBCAM-RI), Yaounde, Cameroun, 3Reproductive, Maternal, Newborn and Child Health (ReMARCH) Research Unit, Yaounde, Cameroon, 4Pediatric, Obstetrics and Gynecology Hospital of Douala, Douala, Cameroon
&Corresponding author
Introduction: extended-spectrum ß-lactamase-producing Enterobacterales (ESBL-E) are an important threat in hospital and community settings. This study aimed to determine the genotypically characterized ESBL-producing Klebsiella pneumoniae (ESBL-Kp) isolated from the stool of children under five years of age with gastroenteritis. The objective was to determine the genotypically characterized ESBL-producing Klebsiella pneumoniae isolated from the stool of children under five years of age with gastroenteritis.
Methods: a prospective and cross-sectional study was carried out at the Mother and Child Center of the Chantal Biya Foundation in Yaoundé. Data were collected through a questionnaire on socio-demographic characteristics. Stool samples were collected from children 0 to 5 years of age and grown on Mac Conkey agar containing purple crystal. Growing colonies were identified with Enterosystem 18R. A double screening for ESBL-Kp was performed by disc diffusion and culture on CHROMagar™ ESBL chromogenic agar. Antimicrobial susceptibility was performed by Kirby-Bauer disc diffusion. Conventional polymerase chain reaction was used to detect genes encoding ESBL production (blaCTX-M, blaTEM, blaSHV).
Results: the prevalence of ESBL-Kp in children was 89.83% (53/59). The majority of isolates were resistant to cefuroxime (98.11%%), amoxicillin-clavulanic acid (96.22%), ceftriaxone (90.56%), cefotaxime (88.67%) and ceftazidime (83.01%). However, most isolates were sensitive to chloramphenicol ( 75.47%), followed by ciprofloxacin (69.81%), levofloxacin (62.26%), and tobramycin (60.37%). A 60.37% prevalence of ESBL-Kp were concomitantly multidrug resistant. The prevalence of antibiotic resistance genes was high for all tested ß-lactamase genes including blaCTX-M (92.45%) and blaTEM (84.90%) compared to blaSHV (77.35%).
Conclusion: the high prevalence of ESBL-Kp and multidrug-resistant Kp among children under five evidenced in this study is concerning and particularly unexpected for this vulnerable population. These results suggest irrational use of antibiotics and underscore the need for improved routine surveillance, antimicrobial stewardship, and infection prevention measures in hospital and community settings to reduce the spread of these resistant bacteria and potential adverse outcomes.
