Conference abstract
Evaluation of the preventive effect of the aqueous extract of Artabotrys thomsonii leaves against insulin resistance and impaired liver function induced by dexamethasone in rats
Pan African Medical Journal - Conference Proceedings. 2023:19(22).23
Nov 2023.
doi: 10.11604/pamj-cp.2023.19.22.2083
Archived on: 23 Nov 2023
Contact the corresponding author
Keywords: Dexamethasone, insulin resistance, oxidative stress, Artabotrys thomsonii
Oral presentation
Evaluation of the preventive effect of the aqueous extract of Artabotrys thomsonii leaves against insulin resistance and impaired liver function induced by dexamethasone in rats
Pechi Fotso Kevin-Armel1,&, Tchamadeu Marie Claire1, Emambo Patience1, Kamdoum Talla Ronald Konrad1, Tene Zogang Christian1, Bogning Zangueu Calvin1, Longo Frida2, Dongmo Alain Bertrand1
1Laboratory of Biology and Physiology of Animal Organisms, Faculty of Sciences of Douala University, P.O. BOX 24157, Douala, Cameroon, 2Laboratory of Animal physiology, Higher Teacher Training College (ENS) of the University of Yaounde1, PoBox 47 Yaoundé, Yaoundé, Cameroon
&Corresponding author
Introduction: Artabotrys thomsonii is a vine of which a plant species of the same genus is traditionally used to cure diabetes mellitus. The present study evaluated the preventive effects of the aqueous extract of the leaves of A. thomsonii on insulin resistance and impaired liver function induced by dexamethasone in rats. The purpose was to evaluate the preventive effect of the plant extract on insulin resistance and impaired liver function in rats.
Methods: after an acute tolerance test, the effects of the plant extract were evaluated in acute administration on the variation of blood glucose in normal rats and those with carbohydrate overload; then in preventive and prolonged administration (21 days) on the variation of the glycemia, of the body mass and of some serum and antioxidant biochemical parameters, in the rats receiving concomitantly dexamethasone (0.4 mg/kg).
Results: the acute administration of Artabotrys thomsonii extract significantly (p<0.05) decreased blood glucose in normal rats and inhibited postprandial hyperglycemia in those with carbohydrate overload at a dose of 400 mg/kg, compared to the various controls. Administered concomitantly with dexamethasone for 21 days in normal rats, the aqueous extract preserved insulin sensitivity (p<0.05-p<0.01), significantly (p<0.05-p<0.001) improved the serum metabolic (creatinemia, triglyceridemia, total cholesterolemia, LDL and HDL cholesterolemia, ARI, activities of ALAT and ASAT, proteinemia) state of the treated rats compared to the dexamethasone control rats. In addition, the plant extract significantly (p<0.05-p<0.001) improves tissue markers of oxidative stress (MDA, SOD, CAT, and NO) in the liver compared to the dexamethasone control rats.
Conclusion: A. thomsonii could be used within the limits of the doses studied to prevent or improve type 2 diabetes.
Evaluation of the preventive effect of the aqueous extract of Artabotrys thomsonii leaves against insulin resistance and impaired liver function induced by dexamethasone in rats
Pechi Fotso Kevin-Armel1,&, Tchamadeu Marie Claire1, Emambo Patience1, Kamdoum Talla Ronald Konrad1, Tene Zogang Christian1, Bogning Zangueu Calvin1, Longo Frida2, Dongmo Alain Bertrand1
1Laboratory of Biology and Physiology of Animal Organisms, Faculty of Sciences of Douala University, P.O. BOX 24157, Douala, Cameroon, 2Laboratory of Animal physiology, Higher Teacher Training College (ENS) of the University of Yaounde1, PoBox 47 Yaoundé, Yaoundé, Cameroon
&Corresponding author
Introduction: Artabotrys thomsonii is a vine of which a plant species of the same genus is traditionally used to cure diabetes mellitus. The present study evaluated the preventive effects of the aqueous extract of the leaves of A. thomsonii on insulin resistance and impaired liver function induced by dexamethasone in rats. The purpose was to evaluate the preventive effect of the plant extract on insulin resistance and impaired liver function in rats.
Methods: after an acute tolerance test, the effects of the plant extract were evaluated in acute administration on the variation of blood glucose in normal rats and those with carbohydrate overload; then in preventive and prolonged administration (21 days) on the variation of the glycemia, of the body mass and of some serum and antioxidant biochemical parameters, in the rats receiving concomitantly dexamethasone (0.4 mg/kg).
Results: the acute administration of Artabotrys thomsonii extract significantly (p<0.05) decreased blood glucose in normal rats and inhibited postprandial hyperglycemia in those with carbohydrate overload at a dose of 400 mg/kg, compared to the various controls. Administered concomitantly with dexamethasone for 21 days in normal rats, the aqueous extract preserved insulin sensitivity (p<0.05-p<0.01), significantly (p<0.05-p<0.001) improved the serum metabolic (creatinemia, triglyceridemia, total cholesterolemia, LDL and HDL cholesterolemia, ARI, activities of ALAT and ASAT, proteinemia) state of the treated rats compared to the dexamethasone control rats. In addition, the plant extract significantly (p<0.05-p<0.001) improves tissue markers of oxidative stress (MDA, SOD, CAT, and NO) in the liver compared to the dexamethasone control rats.
Conclusion: A. thomsonii could be used within the limits of the doses studied to prevent or improve type 2 diabetes.
