Conference abstract
Oxidized palm oil diets induce obesity and impair fertility in female rat
Pan African Medical Journal - Conference Proceedings. 2023:19(27).23
Nov 2023.
doi: 10.11604/pamj-cp.2023.19.27.2111
Archived on: 23 Nov 2023
Contact the corresponding author
Keywords: Palm oil, diets, obesity, female fertility, female rat
Poster
Oxidized palm oil diets induce obesity and impair fertility in female rat
Kengne Tomutou Ines1,&, Djeumeni Neely Ornela1, Hatho Towo Hyacinthe Dominique1, Nguemo Tetio Joël1, Sontia Zidane Barteze1, Tenezogang Takougan Christian1, Ateba Sylvin1, Wankeu-Nya Modeste1, Massoma Lembe Dieudonné1, Moundipa Fewou Paul2, Watcho Pierre3
1Animal Organisms Biology and Physiology Laboratory, Department of Animal Organisms Biology, Faculty of Sciences, University of Douala, P.O. BOX 24157, Douala, Douala, Cameroon, 2Toxicology and pharmacology Laboratory, Department of Biochemistry, Faculty of Sciences, University of Yaoundé I, P.O. BOX 812, Yaoundé, Yaoundé, Cameroon, 3Animal Physiology and Phytopharmacology Laboratory, Department of Animal Biology, Faculty of Sciences, University of Dschang, P.O. BOX 67, Dschang, Dschang, Cameroon
&Corresponding author
Introduction: the oxidation of palm oil is a culinary practice frequently used in our dishes which contributes to an increase in the prevalence of obesity in our society with negative repercussions on female fertility. This study evaluated the effects of two oxidized palm oil diets on some fertility parameters in female rats.
Methods: thirty-three female rats were divided into 3 groups (n=11) receiving respectively for 125 days a standard diet, diets enriched with 30% of oxidized palm oil (OPD1) and 30% of oxidized palm oil + 10% of sucrose solution (OPD2). Throughout the experimentation, the morphometric parameters were evaluated. After the sacrifice, fertility parameters, relative masses of abdominal and periovarian adipose tissues, serum levels of triglycerides, total cholesterol, HDL-c, LDL-c, estradiol, progesterone, and oxidative stress markers in ovaries were also determined. Moreover, the architecture of the ovaries was examined.
Results: in females fed with the two oxidized palm oil diets compared to the control group, we obtained significant increases in morphometric parameters (BMI, Lee's index, and abdominal circumference), and relative masses of abdominal and periovarian adipose tissue associated with dyslipidemia. Moreover, significant increases in pre-implantation losses, serum estradiol, ovarian MDA and GSH levels, ovarian CAT, and SOD activities coupled with decreases in implantation sites and index, progesterone, and NO levels (p<0.05-0.001) were also obtained. Histological and histomorphometric analyses in ovaries showed compared to the control, an important follicular atresia and folliculogenesis disorders in these animals justifying the previously impaired fertility and biochemical parameters in this study.
Conclusion: these results suggest an unfavorable environment for the nidation and/or the development of an embryo probably due to obesity which caused ovarian dysfunction and reduced the egg’s ability to implant in the uterus.
Oxidized palm oil diets induce obesity and impair fertility in female rat
Kengne Tomutou Ines1,&, Djeumeni Neely Ornela1, Hatho Towo Hyacinthe Dominique1, Nguemo Tetio Joël1, Sontia Zidane Barteze1, Tenezogang Takougan Christian1, Ateba Sylvin1, Wankeu-Nya Modeste1, Massoma Lembe Dieudonné1, Moundipa Fewou Paul2, Watcho Pierre3
1Animal Organisms Biology and Physiology Laboratory, Department of Animal Organisms Biology, Faculty of Sciences, University of Douala, P.O. BOX 24157, Douala, Douala, Cameroon, 2Toxicology and pharmacology Laboratory, Department of Biochemistry, Faculty of Sciences, University of Yaoundé I, P.O. BOX 812, Yaoundé, Yaoundé, Cameroon, 3Animal Physiology and Phytopharmacology Laboratory, Department of Animal Biology, Faculty of Sciences, University of Dschang, P.O. BOX 67, Dschang, Dschang, Cameroon
&Corresponding author
Introduction: the oxidation of palm oil is a culinary practice frequently used in our dishes which contributes to an increase in the prevalence of obesity in our society with negative repercussions on female fertility. This study evaluated the effects of two oxidized palm oil diets on some fertility parameters in female rats.
Methods: thirty-three female rats were divided into 3 groups (n=11) receiving respectively for 125 days a standard diet, diets enriched with 30% of oxidized palm oil (OPD1) and 30% of oxidized palm oil + 10% of sucrose solution (OPD2). Throughout the experimentation, the morphometric parameters were evaluated. After the sacrifice, fertility parameters, relative masses of abdominal and periovarian adipose tissues, serum levels of triglycerides, total cholesterol, HDL-c, LDL-c, estradiol, progesterone, and oxidative stress markers in ovaries were also determined. Moreover, the architecture of the ovaries was examined.
Results: in females fed with the two oxidized palm oil diets compared to the control group, we obtained significant increases in morphometric parameters (BMI, Lee's index, and abdominal circumference), and relative masses of abdominal and periovarian adipose tissue associated with dyslipidemia. Moreover, significant increases in pre-implantation losses, serum estradiol, ovarian MDA and GSH levels, ovarian CAT, and SOD activities coupled with decreases in implantation sites and index, progesterone, and NO levels (p<0.05-0.001) were also obtained. Histological and histomorphometric analyses in ovaries showed compared to the control, an important follicular atresia and folliculogenesis disorders in these animals justifying the previously impaired fertility and biochemical parameters in this study.
Conclusion: these results suggest an unfavorable environment for the nidation and/or the development of an embryo probably due to obesity which caused ovarian dysfunction and reduced the egg’s ability to implant in the uterus.
